Binciken gano samfuran ruwa yana da aikace-aikace da yawa a cikin ilimin kimiyyar rayuwa da kula da muhalli.A cikin wannan aikin, mun ƙirƙira ƙaƙƙarfan hoto mai rahusa wanda ya dogara da ƙarfin waveguide capillaries (MCCs) don ƙayyadaddun ƙaddarar sha.Hanya na gani za a iya ƙarawa sosai, kuma ya fi tsayin tsayin jiki na MWC, saboda hasken da ke warwatse da bangon bangon ƙarfe mai santsi yana iya ƙunshe a cikin capillary ba tare da la'akari da kusurwar abin da ya faru ba.Za'a iya samun abubuwan tattarawa kamar ƙananan 5.12 nM ta amfani da reagents na yau da kullun na chromogenic saboda sabon haɓakawar gani mara layi da saurin samfurin sauyawa da gano glucose.
Photometry ana amfani dashi ko'ina don bincike na samfuran ruwa saboda yawan abubuwan da ake samu na chromogenic reagents da na'urorin optoelectronic semiconductor1,2,3,4,5.Idan aka kwatanta da ƙayyadaddun ƙayyadaddun abubuwan sha na al'ada, ruwa mai waveguide (LWC) capillaries suna nuna (TIR) ta hanyar kiyaye hasken bincike a cikin capillary1,2,3,4,5.Duk da haka, ba tare da ƙarin ci gaba ba, hanyar gani kawai yana kusa da tsayin jiki na LWC3.6, kuma ƙara yawan LWC fiye da 1.0 m zai sha wahala daga rashin ƙarfi mai haske da babban haɗari na kumfa, da dai sauransu 3, 7. Game da la'akari. zuwa tantanin halitta da aka tsara don inganta hanyar gani, iyakar ganowa yana inganta kawai ta hanyar 2.5-8.9.
A halin yanzu akwai manyan nau'ikan LWC guda biyu, wato Teflon AF capillaries (wanda yake da ma'anar refractive kawai ~ 1.3, wanda ya fi ƙasa da na ruwa) da silica capillaries wanda aka lulluɓe da Teflon AF ko fina-finai na ƙarfe1,3,4.Don cimma TIR a tsaka-tsaki tsakanin kayan dielectric, ana buƙatar kayan aiki tare da ƙananan ƙididdiga masu mahimmanci da manyan kusurwoyi masu haske 3,6,10.Game da Teflon AF capillaries, Teflon AF yana numfashi saboda tsarinsa mai laushi3,11 kuma yana iya ɗaukar ƙananan abubuwa a cikin samfuran ruwa.Domin ma'adini capillaries rufi a waje tare da Teflon AF ko karfe, da refractive index na ma'adini (1.45) ya fi mafi yawan ruwa samfurori (misali 1.33 na ruwa) 3,6,12,13.Don capillaries mai rufi tare da fim din karfe a ciki, an yi nazarin kaddarorin jigilar kayayyaki14,15,16,17,18, amma tsarin rufewa yana da rikitarwa, farfajiyar fim ɗin ƙarfe yana da tsari mai tsauri da ƙarancin ƙarfi4,19.
Bugu da ƙari, LWCs na kasuwanci (AF Teflon Coated Capillaries da AF Teflon Coated Silica Capillaries, World Precision Instruments, Inc.) suna da wasu rashin amfani, kamar: don kuskure..Babban matattu na TIR3,10, (2) T-connector (don haɗa capillaries, zaruruwa, da bututun shigarwa/kanti) na iya kama kumfa na iska10.
A lokaci guda, ƙaddamar da matakan glucose yana da matukar mahimmanci ga ganewar ciwon sukari, cirrhosis na hanta da rashin lafiyar hankali20.da kuma hanyoyin gano da yawa kamar photometry (ciki har da spectrophotometry 21, 22, 23, 24, 25 da colorimetry akan takarda 26, 27, 28), galvanometry 29, 30, 31, fluorometry 32, 33, 34, 35, spectrophotometry 36 resonance na plasmon.37, Fabry-Perot cavity 38, electrochemistry 39 da capillary electrophoresis 40,41 da sauransu.Koyaya, yawancin waɗannan hanyoyin suna buƙatar kayan aiki masu tsada, kuma gano glucose a cikin adadin nanomolar da yawa ya kasance ƙalubale (misali, don ma'aunin photometric21, 22, 23, 24, 25, 26, 27, 28, mafi ƙarancin taro na glucose).iyakance shine kawai 30 nM lokacin da aka yi amfani da nanoparticles blue na Prussian azaman mimics peroxidase).Ana buƙatar nazarin glucose nananomolar sau da yawa don nazarin matakin salon salula kamar hana haɓakar cutar kansar prostate ɗan adam42 da yanayin daidaitawar CO2 na Prochlorococcus a cikin teku.
Lokacin aikawa: Nuwamba-26-2022